|Method||Product / Instructions||NAPIS Survey Method|
|Visual||Collect symptomatic plant material.||3031 - General Visual Observation|
Detailed guidance on detection survey methodology can be found in the ToBRFV Survey and Response Plan.
No specific signs are present.
The severity and prevalence of symptoms vary based on the age of the plant at the time of infection. However, younger plants typically present fewer symptoms than more mature plants. Symptoms are frequently seen in the apical area of the plant.
Leaves exhibit a mild to severe chlorotic (yellow) mosaic pattern, wrinkling, mottling (blotching), and occasional narrowing.
Infected tomato fruit exhibits yellow and brown discoloration, grooves, deformation, necrosis, and rugosity. The size and number of tomato fruit produced are reduced. In pepper, infected fruits display similar symptoms but can also show green grooves.
Infected calyces, peduncle, sepals, and petioles may also develop necrotic spots that can lead to premature fruit drop. Stems can also show signs of necrosis.
See the CAPS Pest Datasheet for images of symptoms.
The approved method for confirmation is Molecular.
If symptomatic plant material is seen, screen samples onsite using TMV Immunostrips. Alternatively, symptomatic material can be submitted to the authorized diagnostician used by the State for screening. All samples testing positive during screening must be forwarded to the PPQ Beltsville laboratory for confirmatory testing.
Guidance for conducting an immunostrip test and preparing samples for submission can be found in the ToBRFV Survey and Response Plan.
If you are unable to find a reference, contact STCAPS@usda.gov. See the CAPS Pest Datasheet for all references.