Bacterial wilt; Southern bacterial wilt - Ralstonia solanacearum race 3 biovar 2

EEffective: April 6, 2018
Screening Aids:
Taxonomic Position: Burkholderiales : Ralstoniaceae
Pest Type: Bacteria
This pest is a member of the following lists:

Solanaceous Hosts
These Approved Methods are appropriate for: 2023, 2022, 2021, 2020, 2019, 2018, 2017, 2016, 2015
Major Hosts identified in the Host Matrix:
Pest is vectored by:
Approved Method(s):
Survey Instruction Details:
Key Diagnostics

Mistaken Identities:
In Progress / Literature-based Diagnostics:
  1. Caruso, P., Bertolini, E., Cambra, M., and Lopez, M.M. 2003. A new and sensitive co-operational polymerase chain reaction (Co-PCR) for a rapid detection of Ralstonia solanacearum in water. J. Microbiol. Methods 55: 257-272.
  2. Cooke, D., and Sequeira, L. 1991. The use of subtractive hybridization to obtain a DNA probe specific for Pseudomonas solanacearum race 3. Mol. Gen. Genet. 227: 401-410.
  3. Dittanpongpitch, V., and Surat, S. 2003. Detection of Ralstonia solanacearum in soil and weeds from commercial tomato fields using immunocapture and polymerase chain reaction. J. Phytopathology 151: 239-246.
  4. Fegan, M., Holoway, G., Hayward, A.C., and Timmis, J. 1998a. Development of a diagnostic test based on the polymerase chain reaction (PCR) to identify strains of R. solanacearum exhibiting the biovar 2 genotype. In: Bacterial Wilt Disease: Molecular and Ecological Aspects. P. Prior, C. Allen, and J. Elphinstone (Eds.). Springer-Verlag, Berlin. Pp. 34-43.
  5. Fegan, M., Taghavi, M., Sly, L. I., and Hayward, A. C. 1998b. Phylogeny, diversity, and molecular diagnostics of Ralstonia solanacearum. In: Bacterial Wilt Diseases: Molecular and Ecological Aspects. P. Prior, C. Allen, and J. Elphinstone, eds. Springer-Verlag, Berlin. Pages 19-33.
  6. Glick, D.L., Coffey, C.M., and Sulzinski, M.A. 2002. Simultaneous PCR detection of two major bacterial pathogens of geranium. J. Phytopathol. 159: 54-59.
  7. Kubota, R., Vine, B.G., Alvarez, A.M., and Jenkins, D.M. 2008. Detection of Ralstonia solanacearum by loop-mediated isothermal amplification. Phytopathology 98: 1045-1051.
  8. Marco-Noales, E., Bertolini, E., Morente, C., and Lopez, M.M. 2008. Integrated approach to detection of nonculturable cells of Ralstonia solanacearum in asymptomatic Pelargonium spp. cuttings. Phytopathology 98: 949-955.
  9. Opina, N., Tavner, F., Holloway, G., Wang, J.-F., Li, T.-H., Maghirang, R., Fegan, M., Hayward, A.C., Krishnapillai, V., Hong, W.F., Holloway, B.W., and Timmis, J.N. 1997. A novel method for development of species and strain specific DNA probes and PCR primers for identifying Burkholderia solanacearum (formerly Pseudomonas solanacearum). Asia Pacific Journal of Molecular Biology and Biotechnology 5(1): 19-30.
  10. Ozakman, M., and Schaad, N.W. 2003. A real-time BIO-PCR assay for detection of Ralstonia solanacearum race 3, biovar 2, in asymptomatic potato tubers. Canadian Journal of Plant Pathology 25: 232-239.
  11. Pastrik, K.H., and Maiss, E. 2000. Detection of Ralstonia solanacearum in potato by polymerase chain reaction. J. Phytopathol. 148: 619-626.
  12. Poussier, S., and Luisetti, J. 2000. Specific detection of biovars of Ralstonia solanacearum in plant tissues by nested-PCR-RFLP. European Journal of Plant Pathology 106: 255-265.
  13. Pradhanang, P.M., Elphinistone, J.G., and Fox, R.T.V. 2000. Sensitive detection of Ralstonia solanacearum in soil: a comparison of different detection methods. Plant Pathology 49: 414-422.
  14. Schonfeld, J., Heuer, H., van Elsas, J.D., and Smalla, K. 2003. Specific and sensitive detection of Ralstonia solanacearum in soil on the basis of PCR amplification of flicC fragments. Applied and Environmental Microbiology 69(12): 7248-7256.
  15. Weller, S.A., Elphinistone, J.G., Smith, N.C., Boonham, N., and Stead, D.E. 2000. Detection of Ralstonia solanacearum strains with a quantitative multiplex, real-time, fluorogenic PCR (TaqMan) assay. Applied and Environmental Microbiology 66(7): 2853-2858.