Mango sudden decline - Ceratocystis manginecans
Effective: April 8, 2015 - December 31, 2020
Microascales : Ceratocystidaceae
Pest Code (NAPIS):
This pest is a member of the following lists:
These Approved Methods are appropriate for:
2020, 2019, 2018, 2017, 2016
Pest is vectored by:
Hypocryphalus mangiferae (bark beetle). This bark beetle vector has been reported to occur in Florida and Puerto Rico.
|| NAPIS Survey Method
|| Collect symptomatic plant material.
3031 - General Visual Observation
No specific signs are present.
Mango: Symptoms on affected trees include discoloration/dark staining of the vascular tissue, gum exudation, galleries of the beetle vector of the pathogen, leaf wilting and ultimately tree death.
The majority of diseased trees develop large, inconspicuous trunk cankers where the bark appears darker than normal. Beneath the affected bark underlying tissues often discolored brown to black.
Morphological: Morphological characteristics of C. manginecans are described in van Wyk et al. (2007).
Note: Ceratocystis manginecans requires isolation prior to screening. Contact the PPQ National Mycology Specialists (PPQNISNTMycology@usda.gov) prior to the start of the survey season. The mycology specialists will provide guidance on how to sample and isolate the fungus and review the laboratory requirements for successful isolation.
The wilt disease caused by this pathogen is very similar to the wilt disease of mango known to occur in Brazil since 1938 (caused by Ceratocystis fimbriata ).
In Progress / Literature-based Diagnostics:
For molecular identification, comparisons of DNA sequence data for the Internal Transcribed Spacer (ITS), beta tubulin, and Transcription Elongation Factor (TEF) 1-alpha gene regions of C. manginecans were used (van Wyk et al., 2007; Tarigan et al., 2011). These comparisons confirmed previous findings based on morphological characteristics (van Wyk et al., 2007).
Rashid et al. (2013) sequenced and analyzed the nuclear encoded ITS1-5.8s -ITS2 rDNA region of C. manginecans. This study of ITS sequences distinguished C. manginecans from other Ceratocystis spp., and it also identified differences in sequences of C. manginecans isolates from Pakistan from C. manginecans isolates from other countries.
The genome of C. manginecans has recently been published (van der Nest et al., 2014). This will provide more insight on this pathogen in the future.