Host samples or extracted nematodes should be sent to Dr. Lynn Carta:
Dr. Lynn Carta, Ph.D.
Research Plant Pathologist
USDA-ARS BARC-W, Bldg. 010A, Rm. 110
10300 Baltimore Avenue, Beltsville, MD 20705
Office phone: 301-504-8787 Lab phone: 301-504-7039
A. Host collection: The well-established methods for obtaining samples of nematodes from living trees are used.
- A stainless steel tube, sharpened at one end, is driven at an angle of 45Ã¯Â¿Â½ at the point selected for sampling. The tube needs to penetrate a minimum of 5 cm (1.97 in.) into the tree trunk. Multiple samples should be taken from an individual palm and samples should be taken from 0.3 to 2.1 meters (1 to 7 ft.) above the soil line.
- An alternative method: Two holes, up to six inches (15 cm) deep, can be drilled per tree at six inches (15-cm) from both ends using a 2.125-inch (5.4-cm), self-feeding wood bit using an electrical or 18-Volt-battery-powered drill. The wood shavings from the two trees are mixed together, and a minimum 200 g of wood shavings are collected as one lab sample.
Bore holes made with the tube should be sealed with silicone (type used to seal bathroom fixtures) to prevent the entry of weevils, because it is easy to control, inert, and creates a good seal. Holes can also be filled with wooden dowels.
B. Vector collection: Please follow Protocol for Preparing and Forwarding Suspect South American Palm Weevil from Survey Traps for Confirmation and to Maximize Red Ring Nematode Detection