Pale cyst nematode - Globodera pallida
Effective: August 18, 2010
Tylenchida : Heteroderidae
Pest Code (NAPIS):
This pest is a member of the following lists:
This datasheet represents an Approved Method for:
2015, 2016, 2017, 2018, 2019, 2020
Major Hosts identified in the CAPS Host Matrix*:
*This list includes important economic or environmental hosts but does not represent all major hosts of the pest. Check CAPS pest datasheet for complete list of hosts.
Pest is vectored by:
This pest is is not known to be a vector and it is not vectored by any organism.
|| NAPIS Survey Method
| Soil Sample
|| Send sample to nematology diagnostic lab where nematodes will be extracted and identified (preferred method).
3012 - General Soil Sample
| Tissue Sample
|| Send sample to nematology diagnostic lab where nematodes will be extracted and identified.
3011 - General Tissue Sample
Survey Instruction Details:
Laboratory methods that are acceptable for cyst extraction include sugar centrifugation, USDA cyst extractor, Fenwick can sieving, wet sieving, and elutriation.
Mature females and cysts are just visible to the naked eye and can be seen as minute white globes on the root surface.
At the appropriate stage of development, females are prolonged white (slightly cream but no yellow phase) (compare to G. rostochiensis).
Patches of poor growth, chlorosis, and wilting with poor top growth may be seen. Affected plants suffer yield loss, and tubers are smaller.
Morphological: Characteristics of second-stage juveniles, females, and cysts (Baldwin and Mundo-Ocampo, 1991).
Molecular: Immunological, protein, and DNA-based techniques are available. RAPD and RFLP analyses are also available.
There are PPQ CPHST-validated conventional PCR (Skantar et al., 2007) and real-time PCR methods available to detect G. pallida, G. rostochiensis, and G. tabacum (Nakhla et al., 2008).
A real-time PCR has been developed by Nakhla et al. (2010) that shortens the time needed. A validated work instruction is available.
Other Globodera species, particularly Globodera rostochiensis and G. tabacum.
In Progress / Literature-based Diagnostics:
A multiplex PCR was developed to distinguish G. pallida and G. rostochiensis (Pylypenko et al., 2005).
Madani et al. (2005) developed a real-time PCR assay that will rapidly detect and quantify G. pallida and Heterodera schachtii (beet cyst nematode).
Madani et al. (2008) developed a multiplex real-time PCR that can distinguish G. pallida, G. rostochiensis, and G. tabacum.
All three species of Globodera (pallida, rostochiensis, and tabacum) occur in the United States. G. pallida is present in Idaho.
Protocols for processing soil samples for PCN are available.
A synergistic relationship between Verticillium wilt (Verticillium dahliae) and G. pallida has been observed with some potato cultivars.
Contact Ashlee Barth for the most up-to-date work instructions.