Golden nematode - Globodera rostochiensis
Effective: August 18, 2010
Tylenchida : Heteroderidae
Pest Code (NAPIS):
This pest is a member of the following lists:
These Approved Methods are appropriate for:
2022, 2021, 2020, 2019, 2018, 2017, 2016, 2015
Major Hosts identified in the Host Matrix:
This list includes important economic or environmental hosts but does not represent all major hosts of the pest. Check CAPS pest datasheet for complete list of hosts.
Pest is vectored by:
This pest is is not known to be a vector and it is not vectored by any organism.
|| NAPIS Survey Method
| Soil Sample
|| Send sample to nematology diagnostic lab where nematodes will be extracted and identified (preferred method).
3012 - General Soil Sample
| Tissue Sample
|| Send sample to nematology diagnostic lab where nematodes will be extracted and identified.
3011 - General Tissue Sample
Survey Instruction Details:
Laboratory methods that are acceptable for cyst extraction include sugar centrifugation, USDA cyst extractor, Fenwick can sieving, wet sieving, and elutriation.
Mature females and cysts are just visible to the naked eye and can be seen as minute yellow globes on the root surface.
At the appropriate stage of development, females change from white to yellow in G. rostochiensis (compare to G. pallida).
Patches of poor growth, chlorosis, and wilting with poor top growth may be seen. Affected plants suffer yield loss, and tubers are smaller.
Morphological: Characteristics of second-stage juveniles, females, and cysts (Baldwin and Mundo-Ocampo, 1991).
Molecular: Immunological, protein, and DNA-based techniques are available. RAPD and RFLP analyses are also available.
There are PPQ CPHST-validated conventional PCR (Skantar et al., 2007) and real-time PCR methods to detect G. pallida, G. rostochiensis, and G. tabacum (Nakhla et al., 2008).
A real-time PCR has been developed by Nakhla et al. (2010), that shortens the time needed. A validated work instruction is available.
Other Globodera species, particularly Globodera pallida and G. tabacum.
In Progress / Literature-based Diagnostics:
A multiplex PCR was developed to distinguish G. pallida and G. rostochiensis (Pylypenko et al., 2005).
Madani et al. (2008) developed a multiplex real-time PCR that can distinguish G. pallida, G. rostochiensis, and G. tabacum.
A real-time PCR method has been developed for G. rostochiensis (Toyota et al., 2008).
All three species of Globodera (pallida, rostochiensis, and tabacum) occur in the United States. G. rostochiensis is present in New York.
Protocols for processing soil samples for PCN are available.
The golden nematode program manual is available.
Contact Ashlee Barth for the most up-to-date work instructions.