Tomato black ring (TBRV) - Nepovirus Tomato Black Ring Virus

Effective: April 6, 2018
Taxonomic Position: Picornavirales : Secoviridae
Pest Type: Viruses
No manual – See Host Matrix
These Approved Methods are appropriate for: 2020, 2019, 2018, 2017
Pest is vectored by:
Approved Method(s):
Survey Instruction Details:
Key Diagnostics
Approved Method(s):

Mistaken Identities:
In Progress / Literature-based Diagnostics:
  1. Šneideris, D., and J. Staniulis. 2014. Phylogenetic analysis of Lithuanian tomato black ring virus isolates. Žemdirbysté (Agriculture) 101 (2): 193-198.
  2. Šneideris, D., I. Zitikaitë, M. Žižytë, B. Grigali¨naitë, and J, Staniulis. 2012. Identification of nepoviruses in tomato (Lycopersicon esculentum Mill.). Žemdirbysté (Agriculture) 99 (2): 173178.
  3. Digiaro, M., T. Elbeaino, and G. P. Martelli. 2007. Development of degenerate and species-specific primers for the differential and simultaneous RT-PCR detection of grapevine-infecting nepoviruses of subgroups A, B and C. J. Virol. Methods 141: 34-40.
  4. Harper, S. J., C. Delmiglio, L. I. Ward, and G. R. Clover. 2011. Detection of Tomato black ring virus by real-time one-step RT-PCR. Journal of Virological Methods: 171(1): 190-194.
  5. Harrison, B. D., W. P. Mowat, and C. E. Taylor. 1961. Transmission of a strain of tomato black ring virus by Longidorus elongatus (Nematoda). Virology 14: 480-485.
  6. Hasiów-Jaroszewska, B., D. Budzyńska, N. Borodynko, and H. Pospieszny. 2015. 2015. Rapid detection of genetically diverse tomato black ring virus isolates using reverse transcription loop-mediated isothermal amplification. Archives of virology 160(12): 3075-3078.
  7. Martelli, G. P. and C. E. Taylor. 1990. Distribution of viruses and their nematode vectors. Advances in Disease Vector Research. K. F. Harris. New York, Springer-Verlag, p151-189.
  8. Martin, R. R., and I. E. Tzanetakis. 2006. Characterization and recent advances in detection of strawberry viruses. Plant Disease 90: 384-396.
  9. Menzel, W., W. Jelkmann and E. Maiss. 2002. Detection of four apple viruses by multiplex RT-PCR assays with co-amplification of plant mRNA as internal control. J. Virol. Methods 99:81-92.
  10. Pospieszny, H., N. Borodynko, and M. Jonczyk. 2004. First report of Tomato black ring virus (TBRV) in the natural infection of Sambucus nigra in Poland. Journal of Plant Protection Research 44(4): 373-376.
  11. Tzanetakis, I. E. and R. R. Martin. 2013. Expanding field of strawberry viruses which are important in North America. International Journal of Fruit Science 13: 184-195.
  12. Wei, T., and G. Clover. 2008. Use of primers with 5_ non-complementary sequences in RT-PCR for the detection of nepovirus subgroups A and B. J. Virol. Methods 153: 16-21.