There are several fungal pathogens of mango which can be easily confused for N. mangiferae. For example, Lasiodiplodia theobromae, Neofusicoccum parvum, and Fusicoccum aesculi are all anamorphs of Botryosphaeriaceae species which are causal agents of mango stem-end rot and fruit rot. These three pathogens are present in South Africa and Taiwan, two countries known to have N. mangiferae. In addition, numerous other fungal pathogens are known to infect mango, including: Phomopsis spp., Colletotrichum gloeosporioides, Fusarium spp, Pestalotiopsis spp., Alternaria spp, Guignardia spp., Cladosporium spp., Ceratocystis spp, and Botrytis spp. (Ni et al., 2012).
Several other species of Botryosphaeria have also been reported on avocado, including N. parvum, Fusicoccum aesculi, and Dothiorella aromatic (Ni et al., 2009).
In Progress / Literature-based Diagnostics:
To confirm the identity of the fungus, PCR amplification by universal primers, ITS1/ITS4, and DNA sequencing of the internal transcribed spacer (ITS1-5.8S-ITS2 rRNA gene cluster) were conducted. The internal transcribed spacer (ITS) sequence of ribosomal DNA of this fungus was analyzed (Ni et al. (2010).
Ni et al. (2012) developed a nested PCR which can distinguish N. mangiferae from other Botryosphaeriaceae species.
Serrato-Diaz et al., (2014) describe a molecular method for identification by PCR amplification and sequencing of the Internal Transcribed Spacer (ITS) rDNA.